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. 2013 Jan 8;8(1):e53264. doi: 10.1371/journal.pone.0053264

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© 2013 Dong et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Antibody A10 was immobilized on the surface of microplate and then parasites was added. After washing, Fab-H3 displayed phage was added and A10, parasite, phage-H3 form a complex, and immobilized on the surface by A10. HRP/anti-M13 monoclonal antibody conjugate was used to detect the complex. (A) Schematic diagram for detection of parasites. (B) Correlation between parasite and Fabs with a correlation factor of 0.9902 (n = 3). Bovine serum albumin (BSA) was used as a negative control.