Table 2. Results from de novo assembly and annotation per tissue (red or white muscle).
RED MUSCLE | WHITE MUSCLE | |||
Number | % of total | Number | % of total | |
Total numbers | ||||
Contigs (≥100 nt) | 149,159 | 118,572 | ||
Contigs ≥200 nt | 31,609 | 21.2 | 32,061 | 27.0 |
Contigs ≥500 nt | 6,512 | 4.4 | 5,977 | 5.0 |
Maximum contig length (nt) | 15,779 | 16,748 | ||
Number of contigs differentially expressed by RPKM | ||||
Contigs down-regulated in swimmers vs. resters (fc ≤0.5) | 14,932 | 10.0 | 14,928 | 12.6 |
Contigs up-regulated in swimmers vs. resters (fc ≥2) | 21,172 | 14.2 | 12,167 | 10.3 |
Exercise specific contigs | 1,366 | 0.9 | 1,361 | 1.1 |
Non exercise specific contigs | 1,503 | 1.0 | 1,185 | 1.0 |
Contigs annotation accuracy and origin | ||||
Sum of well annotated contigs | 66,035 | 44.3 | 61,437 | 51.8 |
Annotation from SIGENAE salmonid ESTs | 46,785 | 31.4 | 41,696 | 35.2 |
Annotation from zebrafish RefSeq proteins | 8,649 | 5.8 | 9,104 | 7.7 |
Annotation from Metazoa RefSeq proteins | 10,601 | 7.1 | 10,639 | 9.0 |
Contigs ≥500 nt | ||||
Contigs down-regulated in swimmers vs. resters (fc ≤0.5) | 118 | 1.8 | 71 | 1.2 |
Contigs up-regulated in swimmers vs. resters (fc ≥2) | 51 | 0.8 | 29 | 0.5 |
Numbers and maximum size of assembled contigs, differential contig expression by Reads Per Kilobase (exon model) per Million mapped reads (RPKM) values, iterative BLAST annotation results and differentially expressed contigs ≥500 nt are indicated.