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. 2013 Jan 8;8(1):e53171. doi: 10.1371/journal.pone.0053171

Table 2. Results from de novo assembly and annotation per tissue (red or white muscle).

RED MUSCLE WHITE MUSCLE
Number % of total Number % of total
Total numbers
Contigs (≥100 nt) 149,159 118,572
Contigs ≥200 nt 31,609 21.2 32,061 27.0
Contigs ≥500 nt 6,512 4.4 5,977 5.0
Maximum contig length (nt) 15,779 16,748
Number of contigs differentially expressed by RPKM
Contigs down-regulated in swimmers vs. resters (fc ≤0.5) 14,932 10.0 14,928 12.6
Contigs up-regulated in swimmers vs. resters (fc ≥2) 21,172 14.2 12,167 10.3
Exercise specific contigs 1,366 0.9 1,361 1.1
Non exercise specific contigs 1,503 1.0 1,185 1.0
Contigs annotation accuracy and origin
Sum of well annotated contigs 66,035 44.3 61,437 51.8
Annotation from SIGENAE salmonid ESTs 46,785 31.4 41,696 35.2
Annotation from zebrafish RefSeq proteins 8,649 5.8 9,104 7.7
Annotation from Metazoa RefSeq proteins 10,601 7.1 10,639 9.0
Contigs ≥500 nt
Contigs down-regulated in swimmers vs. resters (fc ≤0.5) 118 1.8 71 1.2
Contigs up-regulated in swimmers vs. resters (fc ≥2) 51 0.8 29 0.5

Numbers and maximum size of assembled contigs, differential contig expression by Reads Per Kilobase (exon model) per Million mapped reads (RPKM) values, iterative BLAST annotation results and differentially expressed contigs ≥500 nt are indicated.