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. 2013 Jan 8;8(1):e53338. doi: 10.1371/journal.pone.0053338

Figure 3. Photoreceptor cell apoptosis in primary retinal cell cultures with blood clots.

Figure 3

(A) Schematic image of the double chamber co-culture system of primary retinal cells and blood clots. (B) and (C) The viability of primary retinal cells in the lower chamber was accessed by calcein AM or MitoTracker CMTMRos after 24 h of culture with addition of a clot in the upper chamber (calcein in green, CMTMRos in red, recoverin in blue). The frequency of calcein+ or CMTMRos+ photoreceptors significantly decreased after incubation with clots. Apyrase treatment significantly rescued photoreceptors. (D) The ATP levels of culture medium in the lower chamber were significantly increased by clot exposure, and reversed by apyrase treatment. (E) The ATP levels in plasma and blood. n = 10 per group; **P<0.01. Scale bar: 20 µm.