Figure 4. FGFR signaling increases the activation and nuclear localization pSer-552 β-catenin in hepatoblast and Mat1a .

^−/− cells. Immunofluorescence staining and quantification for pSer-552 β-CATENIN in rFGF7/10-treated MACS-enriched day-4 CD133^pos CD49f^pos CD45^neg (A, B) and Mat1a^−/− cells (C, D). Total number of pSer-552 β-CATENIN positive cells were counted in 3–4 HPF from three independent experiments each and represented as % of total cells (n = 3, *p<0.001). Scale bar 25 µm. (E, F) Western blot analysis of nuclear extracts from Mat1a^−/− cells treated with rFGF10. HISTONE-H3 was used to normalize for relative nuclear protein expression levels (n = 3, *p< 0.005).