Fig. 5.

Quantitative analysis of gene expression of anthocyanin biosynthetic genes through C. gracilis petal development. Photos above the graphs show a representative petal for each of the four developmental stages defined for the purpose of this experiment. From L to R: basal, central, and unspotted. All buds are shown in the upright position (apex at the top, base at the bottom). From left to right, basal spotted plants: spot first appearing, spot well defined, background beginning to show, mature petal. Central spotted plants: no color, spot first appearing, spot well formed, background color beginning to appear. Unspotted plants: young colorless, older colorless, background first appearing, mature petal. Y-axes show expression of genes normalized to Actin. F3′h, flavanoid 3′ hydroxylase; F3′5′h, flavonoid 3′-5′ hydroxylase; Dfr, dihydroflavonol-4-reductase. Columns represent averages across three replicates (except last time point for central spotted, n=2), error bars indicate standard error.