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. Author manuscript; available in PMC: 2014 Jan 23.
Published in final edited form as: J Mol Biol. 2012 Nov 8;425(2):411–423. doi: 10.1016/j.jmb.2012.10.019

Figure 4.

Figure 4

Effects of quercetagetin on UVB-induced JNKs and PI3-K signaling. A and B, quercetagetin inhibits UVB-induced phosphorylation of c-Jun, AKT, and GSK3β, but not JNKs, ERKs, p90RSK, p38, or MSK1. The data are representative of three independent experiments that gave similar results. C, quercetagetin inhibits UVB-induced AP-1 and NF-κB transactivation. Luciferase activity was assayed and AP-1 and NF-κB activities calculated relative to the values for control cells (without UVB). Data are presented as means ± S.D. of AP-1 and NF-κB luciferase activities obtained from three independent experiments. D, quercetagetin inhibits RasG12V-induced focus formation. The data are representative of three independent experiments that gave similar results. The graph shows the average number of foci. E, effects of quercetagetin on H-Ras-induced cell transformation in untreated control cells (a), and cells treated with quercetagetin at a concentration of 5 μM (b), 10 μM (c), or 20 μM (d). Data are presented as means ± S.D. of three independent experiments. The asterisk (*) indicates a significant difference (p < 0.05) between groups untreated or treated with quercetagetin. For D and E, cell colonies were counted under a microscope with the aid of Image-Pro Plus software (v. 4).