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. Author manuscript; available in PMC: 2014 Jan 8.
Published in final edited form as: Biochemistry. 2012 Dec 20;52(1):115–124. doi: 10.1021/bi3009483

Figure 3. Mnn11 limits Kss1 activation through the filamentous growth pathway and not the pheromone response pathway.

Figure 3

Activation of Kss1 and Hog1; mnn11Δ and the indicated double gene deletion mutant cells were stimulated with 0.5 M KCl for 5 min. Cell lysates were resolved by 10% SDS-PAGE and immunoblotting. Specific antibodies were used to detect the dually phosphorylated, fully activated forms of Kss1 and Hog1 (p-Kss1 and p-Hog1). G6PDH served as a loading control. Data are representative of three individual experiments.