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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Nat Med. 2012 Dec 23;19(1):74–82. doi: 10.1038/nm.3040

Figure 5. Endothelial miR-424 and miR-503 regulate PAEC proliferation and migration and induce paracrine inhibition of PASMC proliferation.

Figure 5

(a) Cell cycle analysis of PAECs with miR-424 and miR-503 overexpression in normal and PAH PAECs. (b) Proliferation of normal and PAH PAECs with overexpression of miR-424 and miR-503 or with concurrent stimulation with exogenous FGF2 and transfection of FGFR1 expression construct. *P < 0.01. (c) Proliferation of normal PAECs and PAH PAECs in response to inhibition of miR-424 and miR-503 by anti-miR transfection or with concurrent knockdown of FGF2 and FGFR1. *P < 0.01. (d) Cell migration response to miR-424 and miR-503 overexpression or with concurrent FGF2 stimulation and FGFR1 trasfection in normal PAECs. *P < 0.05 vs. controls. Scale bar = 500 μm. (e) Cell migration response to overexpression of miR-424 and miR-503 in PAH PAECs. *P < 0.05. Scale bar = 500 μm. (f) PASMC proliferation in response to conditioned media (CM) from PAECs of a normal donor and a PAH subject (Control vs. Basal), CM from respective PAECs transfected with miR-424 and miR-503 mimics, and co-transfected with FGF2 expression construct. *P < 0.01. (g) PASMC proliferation in response to CM from PAECs subjected to either APLN knockdown alone or in conjunction with miR-424 and miR-503 overexpression. *P < 0.05. (h) Proliferation of PASMCs in response to CM from PAECs subjected to APLN knockdown, FGF2 knockdown, or concurrent knockdown. *P < 0.05.