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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Nat Struct Mol Biol. 2012 Dec 2;20(1):59–66. doi: 10.1038/nsmb.2431

Figure 1.

Figure 1

Artificial mimics of ATG12~ATG5 do not promote LC3 lipidation. (a) Western blots of LC3-II formation assays carried out in Atg5−/− MEFs stably transduced with the pMX-puro retrovial vectors expressing the indicated 3×FLAG-tagged constructs under non-starvation (−) and starvation (+) conditions. (b) SDS-PAGE of in vitro LC3 lipidation assays carried out with the indicated ATG12~ATG5 conjugate mimics complexed with ATG16N.