Figure 2.

Interaction of hIAPP and SLBs studied by QCM-D. (A and B) QCM-D traces for POPC/POPS (7:3) SLBs exposed to 2 μM hIAPP (A) and 2 μM hIAPP and 100 μM Ca²⁺ ions (B). A comparison of the dissipation curves (ΔD_n) shows that hIAPP interacts less with the hydrophobic core of the SLB in the presence of calcium ions. In the presence of Ca²⁺, a decrease in the mass adsorbed to the quartz crystal at ∼950 min (corresponding to the increase in ΔF_n) indicates the removal of lipid from the bilayer after fiber formation. All experiments were performed in 10 mM HEPES buffer, 100 mM NaCl, pH 7.4 at 25°C.