Table 1.

Experimental G(V) shifts and kinetics data for new mutants

	V1/2 (mV)		${\tau }_{+50\text{mV}}$ (ms)		${\tau }_{-50\text{mV}}$ (ms)
WT	−20.4 ± 0.5	n = 5	1.0 ± 0.1	n = 3	10.1 ± 0.9	n = 3
F290L	+23.2 ± 2.4	n = 13	1.1 ± 0.2	n = 3	<0.5	n = 3
V236A	−2.6 ± 1.1	n = 8	1.1 ± 0.1	n = 8	0.8 ± 0.1	n = 7
I237A	+34.5 ± 5.1	n = 4	1.6 ± 0.2	n = 6	<0.5	n = 6
I287A	+17.7 ± 4.4	n = 3	1.8 ± 0.3	n = 4	<0.5	n = 4
V236A/I287A	no expression
I237A/I287A	+18.4 ± 0.6	n = 3	1.2 ± 0.1	n = 3	<0.5	n = 3

All mutations shift G(V) in a positive direction, and there is very little or no effect on the opening kinetics compared to WT, whereas the closing is accelerated by more than one order of magnitude in all cases, and for several mutants it is too fast to measure, which corresponds to at least a 20-fold speedup.