Figure 4.

T-bet Is Required for Optimal Expression of IFN-γ by Intestinal ILCs, and in the Absence of T-bet ILCs Selectively Produced IL-17A

(A) Cytokine concentrations in ex vivo explant cultures from Rag2^−/− (n = 5) and TRnUC (n = 5) mice following gavage with H. typhlonius, measured by ELISA. Results show mean, and error bars represent SEM.

(B) Intracellular cytokine expression in live CD45⁺CD90^hi cLP ILCs from Rag2^−/− and TRnUC mice in H. typhlonius-associated colitis. Cells were stimulated with PMA and ionomycin.

(C) Organ mass in Rag2^−/− mice following treatment with agonistic CD40 mAbs or control antibody. Also see Figure S4A for other clinical features.

(D) Flow cytometry histogram demonstrating the proportion of Gr-1^hi granulocytes in the cLP of Rag2^−/− mice following anti-CD40 or control mAb administration.

(E) Representative flow cytometry plot showing intracellular cytokine and T-bet expression in lin⁻CD90⁺ ILCs from mLN of Rag2^−/− and TRnUC mice following CD40 mAb treatment.

(F) Proportions of IL-17A⁺ or IFN-γ⁺CD90⁺ ILCs in the cLP of Rag2^−/− or TRnUC mice following administration of anti-CD40.