Figure 4.

Regulation of miR-25 Levels Strongly Sensitizes Cells to Ca²⁺-Dependent Apoptotic Stimuli

(A) [Ca²⁺]_m peaks in PC3 cells: Ctrl miR: 5.25 ± 0.59 μM; anti-miR-25: 7.81 ± 0.64 μM. n = 16 independent experiments.

(B) [Ca²⁺]_m peaks in HCT116 cells: Ctrl miR: 2.28 ± 0.21 μM; anti-miR-25: 3.32 ± 0.31 μM. n = 16 independent experiments.

(C and D) Microscopy counts of cell viability in PC3 (C) and HCT116 (D) cells. Treatments with H₂O₂ (500 μM for 2 hr) and C2-ceramide (C2-cer.; 40 μM for 2 hr) reveal a more efficient apoptosis induction after anti-miR-25 transfection. n = 3 independent experiments.

(E and F) Immunoblot shows increased levels of cleaved PARP and cleaved caspase-3 in anti-miR-25-expressing PC3 (E) and HCT116 (F) cells after treatment with C2-ceramide (C2-cer.; 40 μM for 2 hr).

^∗p < 0.05; error bars correspond to mean ± SEM. See also Figure S4.