Table 2.
Expression of microinjected cRNA encoding luciferase-tagged wild-type phospholipase Cζ (PLCζ) mutants PLCζH233L and PLCζH398P in unfertilized mouse eggs.
| PLCζ-luciferase injected | Ca2+oscillations (spikes/2 h) | Peak luminescence (cps) | Time to 1st spike (min) | Luminescence at 1st spike (cps) |
|---|---|---|---|---|
| PLCζWT | 9.02 ± 0.037 | 0.42 ± 0.020 | ∼25 | 0.07 ± 0.005 |
| PLCζH233L | 2.84 ± 0.076 | 0.40 ± 0.050 | ∼190 | 0.34 ± 0.040 |
| PLCζH398P | — | 0.39 ± 0.020 | — | — |
Note: Values are mean ± standard error of the mean. The Ca2+ oscillation-inducing activity (Ca2+ spike number in 2 hours; time to first spike) and the simultaneously-measured PLCζ-luciferase fusion protein luminescence levels (peak luminescence; luminescence at first spike) are summarized for mouse eggs that had been microinjected, as described in Materials and Methods, with cRNA encoding one of the following human PLCζ-luciferase constructs: wild-type PLCζ, the PLCζH233L or PLCζH398P mutant (see Fig. 4B). cps = counts per second.