Table 1. Comparative analysis of hMSCs and hMAPCs.
| Stem cell | hMSCs | hMAPCs |
|---|---|---|
| Isolation and culture | ||
| Source | Bone marrow | Bone marrow/bone |
| % Serum in expansion medium | 10% | 2% |
| Extra growth factors in expansion medium | / | PDGF/EGF |
| Plating density | 5000 cm−2 | 400 cm−2 |
| % Oxygen | 21% | 5% |
| Cell surface phenotype | ||
| CD34 | negative | negative |
| CD45 | negative | negative |
| c-kit | negative | negative |
| KDR | negative | negative |
| CD56 | negative | negative |
| CD271 | negative | negative |
| CD146 | low | low |
| CD44 | high | high |
| CD13 | high | high |
| CD73 | high | high |
| CD90 | high | high |
| CD105 | high | high |
| MHC class I | high | low |
| CD140a | low | negative |
| CD140b | high | negative |
| ALP | low | negative |
| Proliferation capacities | ||
| Population doublings | 20–30 | 70 |
| Differentiation potential | ||
| Adipocytes/osteoblasts/chondrocytes | yes | yes |
| Smooth muscle cells | yes | yes |
| Endothelial cells | no | yes |
Abbreviations: ALP, alkaline phosphatase; EGF, epidermal growth factor; hMAPC, human multipotent adult progenitor cell; hMSC, human mesenchymal stem cell; KDR, kinase insert domain receptor; MHC, major histocompatibility complex; PDGF, platelet-derived growth factor.