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. 2013 Jan 9;3:1045. doi: 10.1038/srep01045

Figure 3. Knocking down vimentin expression in human macrophages increases production of ROS.

Figure 3

(A) Western blot confirming knockdown of vimentin expression in the human monocytic cell line THP-1 transduced with shRNA targeting vimentin. (B). Fluorescence microscopy demonstrating levels of ROS detected by DCFH-DA in PMA-stimulated THP-1 cells transduced with an empty lentiviral vector (Control shRNA) or an shRNA targeting vimentin (Vim KD). (C). Flow cytometry analysis of the cells described in B. Data represents 2 independent experiments. (D). NADPH levels were measured in the WT human monocytic cell line THP-1, or THP-1 cells transduced with either an empty lentiviral vector (Control) or a vector expressing an shRNA targeting vimentin (Vim KD) after overnight serum deprivation. Data represent 2 independent experiments preformed in triplicate. *P < 0.05 comparing Vim KD cells to the control or wild-type cells.