Figure 3.

Proliferation is not increased with concurrent prednisolone administration. Bromodeoxyuridine (BrdU) staining (red) was used as a measure of proliferation. At baseline (No Dox), there are 1-2 BrdU positive cells per high-power field in the neuroepithelium. With doxycycline-induced TNF-α expression for six weeks (Dox), there are no visible BrdU positive cells even in the small areas of preserved neuroepithelium. In concurrent doxycycline-prednisolone administration (Dox/Prednisolone), areas of preserved neuroepithelium have a similar staining as the wild-type mouse. Scale bar represents 10 μm.