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. 2001 Jun 26;98(14):7700–7705. doi: 10.1073/pnas.131022398

Figure 4.

Figure 4

RP-HPLC analysis of [1-14C]arachidonic acid metabolites of G. fruticosa (A) and P. homomalla (B) COX expressed in Sf9 insect cells. The incubation products were run on a Waters Symmetry C18 column (25 × 0.46 cm) using a solvent of water/acetonitrile/acetic acid (62.5/37.5/0.01, vol/vol/vol, changed to the proportions 30/70/0.01 at 25 min) at a flow rate of 1 ml/min and using a Radiomatic Flo-One detector for on-line recording of radioactivity.