Fig. 1.

TPH2 immunoreactivity (red) is present in neurons of the myenteric plexus of the mouse intestine. Antibodies to HuC/D (blue) are used as a neuronal marker and reveal the cell bodies of all neurons. Double label immunoreactivity demonstrates both antigens in the same sections. Left panel: TPH2 immunoreactivity. Center panel: HuC/D immunoreactivity. Right panel: Merged image. Note that TPH2 is found in 2 neurons (arrows) but not in all and that it also extends into varicose axons running in interganglionic connectives (arrowheads). The marker = 25 μm.