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BMP‐2 increases Smad 1/5/8 activation. Human coronary artery smooth muscle cells were treated with BMP‐2 and phosphorylation of Smad 1/5/8 (p‐Smad 1/5/8) was examined up to 30 minutes. Total levels of Smad 1/5/8 and of the regulatory Smad, Smad 4, were also examined. Representative blots are shown (n=3). BMP‐2 indicates bone morphogenetic protein‐2.
