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. 2012 Dec 19;1(6):e003855. doi: 10.1161/JAHA.112.003855

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© 2012 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley-Blackwell.

This is an Open Access article under the terms of the Creative Commons Attribution Noncommercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Figure 1. — Mitochondrial protein content, but not mass, is decreased in infected cardiomyocytes and chagasic hearts. A and B, Cardiomyocytes were infected with Trypanosoma cruzi for 0 to 24 hours: (A) citrate synthase activity; (B) Western blotting for CYTB, SDHA, and β-actin; (C) cryostat sections of the heart biopsies from normal healthy donors (a, d, g), chagasic patients (b, e, h), and other cardiomyopathy (OCM) patients (c, f, i) were submitted to immunostaining for ND1 (a-c), CYTB (d-f), and CYPA (g-i) (magnification ×20). Data in all bar graphs are presented as mean±SD. Statistical significance of normally distributed data was analyzed by the Student t test/ANOVA/Tukey's test; *P<0.05, **P<0.01, ***P<0.001. In some bar graphs, statistical significance by the nonparametric Kruskal–Wallis test is also presented (horizontal dotted line). SD indicates standard deviation; ANOVA, analysis of variance.