Table 2.
Arbitrary Scores, Mean (Range) | |||
---|---|---|---|
Immunostaining | Normal | Chagasic | Other Cardiomyopathy |
Figure 1 | |||
CYTB | 12.5 (9.3 to 16.8) | 7.4 (1.7 to 16.4)*†† | 22.0 (1.7 to 40.0)* |
ND1 | 54.6 (2.8 to 26.7) | 16.4 (1.5 to 56.5)*‡ | 1.9 (0.2 to 4.6)*§§§ |
CYPA | 141 (45.0 to 452) | 177 (24.1 to 380) | 104 (34.2 to 182) |
Figure 3 | |||
PGC-1α | 1399 (1141 to 1963) | 273.9 (5.7 to 1458.8)**‡§§§ | 792 (156 to 2557)**§ |
PPARγ | 60.1 (3.6 to 369) | 6.0 (0.2 to 20.9)***§§§ | 1.4 (0.5 to 2.2)*§§ |
NRF2 | 1017 (505 to 1798) | 846(112 to 1461)* | 766 (690 to 895) |
Figure 6 | |||
4-HNE | 12.8 (1.3 to 54.3) | 197.4 (53.4 to 817)***‡§§§ | 632 (116 to 1934)**§§§ |
3-NT | 7.3 (2.1 to 14.8) | 416.0 (173.6 to 711.6)***‡§§§ | 260 (4.0 to 1027)**§ |
8-OHdG | 376 (76 to 883) | 725 (437 to 1442)* | 1099.3 (687 to 1442)§ |
Figure 7 | |||
SOD2 | 1228(119 to 4629) | 763 (156 to 3599)* | 1035 (212 to 2651) |
HO-1 | 431(39.4 to 2529) | 4.3 (1.3 to 9.4)*‡§§§ | 111 (5.0 to 527)**§ |
Cardiac biopsies of normal donors and of chagasic and other cardiomyopathy patients (n=6 to 8/group) were fixed in OCT, and 5-μm-thick cryostat sections (5 sections/tissue) were submitted for immunostaining, as described in Materials and Methods. Each slide was analyzed for 5 microscopic fields on an Olympus polarizing microscope (Center Valley, PA), and staining for primary antibody was scored as a percentage of total histological fields quantified using MetaMorph Microscopy Automation & Image Analysis Software (Molecular Devices). The Mann–Whitney test was employed for pairwise comparison.
Significance by t tests is presented as *normal versus disease (chagasic or other cardiomyopathy); ‡chagasic versus other cardiomyopathy.
Significance by the Kruskal–Wallis Dunn's test is presented as §normal versus chagasic; §normal versus other cardiomyopathy; †chagasic versus other cardiomyopathy groups.
Significant at *‡§P < 0.05, **††§§P < 0.01, ***§§§P < 0.001.