Table 2.

Semiquantitative Scoring of Immunostaining of Cardiac Tissue Biopsies

	Arbitrary Scores, Mean (Range)
Immunostaining	Normal	Chagasic	Other Cardiomyopathy
Figure 1
CYTB	12.5 (9.3 to 16.8)	7.4 (1.7 to 16.4)*††	22.0 (1.7 to 40.0)*
ND1	54.6 (2.8 to 26.7)	16.4 (1.5 to 56.5)*‡	1.9 (0.2 to 4.6)*§§§
CYPA	141 (45.0 to 452)	177 (24.1 to 380)	104 (34.2 to 182)
Figure 3
PGC-1α	1399 (1141 to 1963)	273.9 (5.7 to 1458.8)**‡§§§	792 (156 to 2557)**§
PPARγ	60.1 (3.6 to 369)	6.0 (0.2 to 20.9)***§§§	1.4 (0.5 to 2.2)*§§
NRF2	1017 (505 to 1798)	846(112 to 1461)*	766 (690 to 895)
Figure 6
4-HNE	12.8 (1.3 to 54.3)	197.4 (53.4 to 817)***‡§§§	632 (116 to 1934)**§§§
3-NT	7.3 (2.1 to 14.8)	416.0 (173.6 to 711.6)***‡§§§	260 (4.0 to 1027)**§
8-OHdG	376 (76 to 883)	725 (437 to 1442)*	1099.3 (687 to 1442)§
Figure 7
SOD2	1228(119 to 4629)	763 (156 to 3599)*	1035 (212 to 2651)
HO-1	431(39.4 to 2529)	4.3 (1.3 to 9.4)*‡§§§	111 (5.0 to 527)**§

Cardiac biopsies of normal donors and of chagasic and other cardiomyopathy patients (n=6 to 8/group) were fixed in OCT, and 5-μm-thick cryostat sections (5 sections/tissue) were submitted for immunostaining, as described in Materials and Methods. Each slide was analyzed for 5 microscopic fields on an Olympus polarizing microscope (Center Valley, PA), and staining for primary antibody was scored as a percentage of total histological fields quantified using MetaMorph Microscopy Automation & Image Analysis Software (Molecular Devices). The Mann–Whitney test was employed for pairwise comparison.

Significance by t tests is presented as *normal versus disease (chagasic or other cardiomyopathy); ^‡chagasic versus other cardiomyopathy.

Significance by the Kruskal–Wallis Dunn's test is presented as ^§normal versus chagasic; ^§normal versus other cardiomyopathy; ^†chagasic versus other cardiomyopathy groups.

Significant at *^‡§P < 0.05, **^††§§P < 0.01, ***^§§§P < 0.001.