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. 2012 Dec 19;1(6):e002238. doi: 10.1161/JAHA.112.002238

Figure 5.

Figure 5.

In vitro responses of MSCs to selective differentiation media. A, Quantification of MSC differentiation toward adipocytes. B and C, Quantification of PPARγ and adiponectin expression in MSCs. D, Quantification of MSC differentiation toward endothelial cells. E, Quantification of MSC tubular formation capacity. F through H, Quantification of eNOS and P-eNOS and NO levels in MSCs. (For A through H: mean±SEM, n=6; *P<0.05 vs WT, †P<0.05 vs db/db.) I, Representative photomicrographs of adipocyte differentiation (Oil Red O staining, hematoxylin counter stain, ×200). J, Representative photomicrographs of endothelial differentiation (CD31 immunostaining [red]; DAPI counter stain [blue], ×200). K, Representative photomicrographs of tubular structure from each group (×100 magnification). MSCs indicates mesenchymal stem cells; WT, wild-type; eNOS, endothelial NOS; P-eNOS, phosphorylated eNOS; and NO, nitric oxide.