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. 2012 Dec 19;1(6):e005652. doi: 10.1161/JAHA.112.005652

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© 2012 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley-Blackwell.

This is an Open Access article under the terms of the Creative Commons Attribution Noncommercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Figure 7. — Myofibroblasts. Myofibroblasts identified by nonvascular α‐smooth muscle actin staining of the infarct and border zones of sections of myocardium harvested 7 weeks after coronary ligation and administration of AdVEGF‐All6A⁺ or the control vector AdNull (4 weeks after the administration of lentivirus encoding GMT or a GFP control). A, Photomicrographs of representative sections of infarct zones viewed at ×400 after administration of AdNull/GFP (top) or AdVEGF‐All6A⁺/GMT (bottom). Arrows depict cells identified as myofibroblasts. Bars represent 50 μm. B, The number of myofibroblasts identified per microscopic field (×200) in animals receiving GMT versus GFP control vectors (n=12), as identified by staining for α‐smooth muscle actin (P=0.09). GFP indicates green fluorescent protein; GMT, Gata4, Mef2c, and Tbx5; VEGF, vascular endothelial growth factor.