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Alcian blue staining. (a and b) Micromass cultures and (c and d) constructs of silk with hASCs cultured for 6 weeks. Extracellular matrices of proteoglycan (blue) were detected in the both groups. Arrow (→) indicates examples of chondrocyte-specific lacunae. Scale bars indicate 250 µm (a and c; 10×) and 100 µm (b and d; 40×).
