Extracellular levels of glutamate increase but glutamate transporter expression remains unchanged or decreases after sciatic nerve injury. A, Glutamate in the CSF of uninjured (naïve) rats (N=9), and rats 1 week (N=13) or 4 weeks (N=14) after spared nerve injury (SNI). B, Glutamate transport activity measured in membrane preparations of the ipsilateral dorsal horn of naïve rats and rats 1 week after SNI (N=4–6). C, Western blot analysis of Glt1, Glast and Eaac1 expression in the ipsilateral dorsal horn after SNI, N=6 per group. * p<0.05