Figure 3.

Yeast 2-hybrid “one-on-one” assays between GapC isoforms, aldolase, and VDAC3. Putatively positive interactions had been identified in the yeast two-hybrid screen of the cDNA library from A. thaliana. Here, to retest these findings, the haploid yeast strain AH109 was cotransformed with constructs, expressing bait, or prey proteins, as indicated in the table. Serial dilution of the appropriate overnight yeast culture was dropped onto selective media to demonstrate the stringency of binding between proteins. Colonies growing on SD/Leu⁻, Trp⁻ were successfully transformed with both vectors, encoding the investigated proteins. Growth on SD/TDO and SD/QDO/X-α-Gal is a sign of positive interactions. Italics indicate false positive results due to autoactivation by VDAC3 when used as a prey.