Figure 4.

Colocalization of aldolase (FBA6) and GapC with mitochondria. Vectors encoding the investigated proteins were cotransformed in protoplasts, isolated from leaves of A. thaliana plants. The fluorescent signals from GapC1 interacting with VDAC3 (A,B), FBA6, and VDAC3 (C), as well as FBA6 with GapC1 or GapC2 (D,E), fused with N- or C-terminal halves of YFP, respectively, were tested for mitochondrial localization by staining the cells with 50 nM MitoTracker^® Orange CMTMRos (Molecular Probes).