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. 2013 Jan 9;3:284. doi: 10.3389/fpls.2012.00284

Figure 5.

Figure 5

Co-sedimentation assay with F-actin, GAPDH, and aldolase under oxidizing and reducing conditions. Samples with rabbit F-actin, FBA6, and GapC1 were first oxidized with the specific redox reagents(A) Diamide + GSH (each 0.5 mM); (B) GSSG (5 mM);(C) H2O2 + GSH (each 0.5 mM); (D) GSNO (0.5 mM) with a following reduction by DTTred (10 mM). The control sample with F-actin, FBA6, and GapC1 was only incubated with DTTred (10 mM). The mixtures were then centrifugated at 100,000 × g for 1 h. Supernatant (S) and pellet (P) were separated for analysis by SDS-PAGE and the gels were stained with Coomassie Brilliant blue.