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. 2013 Jan 9;3:284. doi: 10.3389/fpls.2012.00284

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Copyright © 2013 Wojtera-Kwiczor, Groß, Leffers, Kang, Schneider and Scheibe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Figure A2 — Occasional colocalization of GAPDH (GapC1) with mitochondria. GapC1:GFP was transiently expressed in the protoplasts, isolated from Arabidopsis plants. Mitochondria were stained using 50 nMMitoTracker^® Orange CMTMRos (Molecular Probes). Two regions of interest (ROI), depicted with a white frame, were magnified in order to perform a colocalization analysis by means of a fluorescence-intensity profile. One ROI displays a possible association of the glycolytic enzyme with mitochondrion (A), whereas the other ROI shows no mitochondria association at the moment of visualization (B).