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Negative controls for BiFC experiments analyzing any colocalization of aldolase (FBA6) and GapC1. Vectors encoding the investigated proteins were cotransformed in protoplasts, isolated from leaves of A. thaliana plants. (A-C) is a time course of the expression of the empty vector with only the N-terminal half of YFP and GapC1 fused with the C-terminal half of YFP. In (D), the same is shown for FBA6 after 4 h.
