TABLE 2.
Sterola | WTb | KD7c | KD21c | 25-TLd | KD7PYc |
1 | 0.3 | 0.7 | 2.5 | 1.1 | |
2 | 0.3 | ||||
3 | 0.3 | ||||
4 | tre | tr | tr | 0.9 | |
5 | tr | tr | tr | tr | tr |
6 | 2.7 | ||||
7 | tr | ||||
8 | 2.6 | 14.9 | 8.1 | 21.4 | |
9 | 1.6 | ||||
10 | 3.5 | 3 | 0.5 | ||
11 | 0.5 | 6.4 | 10.6 | ||
12 | 21.3 | ||||
13 | 0.9 | 4.6 | |||
14 | 50.8 | 36.6 | |||
15 | 70.4 | ||||
16 | 37.2 | 27.5 | 1.5 | ||
17 | |||||
18 | 15.5 | ||||
19 | 0.5 | ||||
20 | 8.8 | ||||
21 | 22.6 | 21.5 | |||
22 | 19.4 | 12.7 | |||
23 | 1.7 | 2.9 | |||
24 | 35 | 26.8 |
Structures of sterols are shown in Fig. 7.
WT, wild-type cells.
Mutant cell lines.
25TL, 25-thialanosterol salt treated cells.
tr, trace amount of sterol at less than 0.3%; blank refers to no sterol detected in cells.