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Enhanced inflammatory responses in SR-BI−/− BMM in response to LTA. BMMs were harvested and differentiated as described in Materials and Methods. BMMs were incubated with LTA (1 μg/ml) in 5% FBS-containing medium for 4 h. Cells were harvested, and RNA was extracted with Trizol-RT. Target gene expression was determined by Q-PCR. Relative values to GAPDH are shown as the mean ± SD of quadruplicate determinations. *P < 0.05, **P < 0.01. (A) TNFα. (B) IL-1β. (C) IL-6. (D) MCP-1.
