Fig. 2.

Expression of selected genes identified as responsive to EO in THP-1 cells. Genes were selected from Table 1 and Fig. 1 for validation by real-time PCR. Cells were treated, and RNA was extracted as described in Methods. Gene expression following treatment with EO (0, 1, 10, 100 μM) is expressed relative to a housekeeping gene (GAPDH) and normalized to vehicle control (DMSO). Asterisks denote significantly different from control (P < 0.05, n = 3). Bars represent mean and SEM.