Fig. 1.

pTWIN1 vector map showing the design of the apoA-II expression construct. Mature human apoA-II (blue) was subcloned into the pTWIN1 expression vector upstream of the T7 promoter with Mxe intein, a chitin binding domain, and a 6× histidine tag on its C terminus (green). To initiate transcription, a ATG codon was added to the N-terminus of the protein sequence. The site of intein-mediated endo-cleavage is indicated by a black arrow. The amino acid sequence of the translated apoA-II-intein fusion protein is reported: mature apoA-II (blue), modified Mxe intein (green).