Fig. 4.

Far UV circular dichroism spectra and Gnd-HCl denaturation. A: Far UV CD spectra of human plasma (solid) and recombinant (dashed) apoA-II. Samples were run at room temperature in triplicate for three accumulations each and reported as the overall mean. B: Isothermal Gnd-HCl denaturation of lipid-free human plasma (closed circles) and recombinant (open circles) apoA-II. Samples at 100 µg/ml were incubated at 4°C with the indicated concentration of Gnd-HCl for 72 h to ensure equilibrium. Average values and error bars from at least two separate experiments are reported.