TABLE 1.
Significant changes in lipid species in SPTLC123 or DEGS1 siRNA-treated cells
| Lipid Class | Lipid Species | Scrambled siRNA | SPTLC123 siRNA | SPTLC123 P | DEGS1 siRNA | DEGS1 P | SPTLC123 versus DEGS1 P |
| Ceramide | Cer 16:0 | 1.000 | −1.469 | NS | −3.408 | P < 0.01 | NS |
| Ceramide | Cer 24:0 | 1.000 | −4.485 | P < 0.001 | −2.560 | P < 0.001 | P < 0.001 |
| Ceramide | Cer 24:1 | 1.000 | −3.428 | P < 0.001 | −2.152 | P < 0.001 | P < 0.001 |
| Ceramide | Cer 26:1 | 1.000 | −8.185 | P < 0.001 | −3.194 | P < 0.01 | NS |
| Dihydroceramide | dhCer 14:0 | 1.000 | −1.799 | NS | 10.072 | P < 0.001 | P < 0.001 |
| Dihydroceramide | dhCer 16:0 | 1.000 | −1.497 | NS | 12.207 | P < 0.001 | P < 0.001 |
| Dihydroceramide | dhCer 18:0 | 1.000 | −1.371 | NS | 8.332 | P < 0.001 | P < 0.001 |
| Dihydroceramide | dhCer 20:0 | 1.000 | 1.268 | NS | 10.512 | P < 0.001 | P < 0.001 |
| Dihydroceramide | dhCer 22:0 | 1.000 | −1.867 | NS | 25.166 | P < 0.001 | P < 0.001 |
| Dihydroceramide | dhCer 22:1 | 1.000 | −4.376 | NS | 9.928 | P < 0.001 | P < 0.001 |
| Dihydroceramide | dhCer 24:0 | 1.000 | −3.427 | NS | 67.891 | P < 0.001 | P < 0.001 |
| Dihydroceramide | dhCer 24:1 | 1.000 | −3.847 | NS | 27.561 | P < 0.001 | P < 0.001 |
| Dihydroceramide | dhCer 26:0 | 1.000 | −1.680 | NS | 25.921 | P < 0.001 | P < 0.001 |
| Dihydroceramide | dhCer 26:1 | 1.000 | −4.689 | NS | 78.955 | P < 0.001 | P < 0.001 |
| Sphinganine | dhSph | 1.000 | −1.041 | NS | 7.490 | P < 0.001 | P < 0.001 |
| Sphingomyelin | SM 16:0 | 1.000 | 1.461 | P < 0.001 | −2.546 | P < 0.001 | P < 0.001 |
| Cardiolipin | CL 16:0 | 1.000 | 1.236 | P < 0.001 | 1.195 | P < 0.001 | NS |
| Cardiolipin | CL 16:1n7 | 1.000 | 1.273 | P < 0.001 | −1.144 | P < 0.01 | P < 0.001 |
| Cardiolipin | CL 18:1n7 | 1.000 | 1.210 | P < 0.001 | −1.038 | NS | P < 0.001 |
| Cholesteryl ester | CE 16:0 | 1.000 | 1.201 | P < 0.05 | 1.429 | P < 0.001 | P < 0.01 |
| Free fatty acid | FFA 16:0 | 1.000 | 1.026 | NS | 1.578 | P < 0.001 | P < 0.001 |
| Free fatty acid | FFA 18:1n9 | 1.000 | −1.156 | NS | 1.878 | P < 0.001 | P < 0.001 |
| Diacylglycerol | DAG 16:0 | 1.000 | −1.413 | NS | 1.613 | P < 0.01 | P < 0.001 |
| Triacylglycerol | TAG 16:1n7 | 1.000 | −1.273 | NS | −2.645 | P < 0.05 | NS |
| Triacylglycerol | TAG 18:1n7 | 1.000 | −1.322 | NS | −2.152 | P < 0.01 | NS |
| Triacylglycerol | TAG 18:1n9 | 1.000 | −1.657 | P < 0.001 | −2.414 | P < 0.001 | P < 0.01 |
| Lysophosphatidylcholine | LYPC 16:0 | 1.000 | 1.703 | P < 0.01 | 1.607 | P < 0.05 | NS |
| Phosphatidylcholine | PC 16:0 | 1.000 | 1.330 | P < 0.001 | −1.016 | NS | P < 0.001 |
| Phosphatidylcholine | PC 16:1n7 | 1.000 | 1.441 | P < 0.001 | −1.283 | NS | P < 0.001 |
| Phosphatidylcholine | PC 18:1n7 | 1.000 | 1.299 | P < 0.01 | −1.334 | NS | P < 0.001 |
| Phosphatidylcholine | PC 18:1n9 | 1.000 | 1.118 | P < 0.01 | −1.097 | NS | P < 0.001 |
| Phosphatidylethanolamine | PE 20:4n6 | 1.000 | 1.434 | P < 0.05 | 1.248 | NS | NS |
| Phosphatidylserine | PS 18:0 | 1.000 | 1.113 | P < 0.01 | 1.059 | NS | NS |
| Phosphatidylserine | PS 20:4n6 | 1.000 | 1.205 | NS | −1.160 | NS | P < 0.05 |
Lipid levels were determined by LC/MS in Huh7 cells treated with scrambled, SPTLC123, or DEGS1 siRNA. The table summarizes lipid species across the different siRNA treatments are expressed as fold-change relative to scrambled siRNA treatment. Statistically significant differences are represented in bold (P ≤ 0.05 after Bonferroni correction). All 289 lipids compared in this study containing the actual values can be found in supplementary File II.
NS, nonsignificant.