TABLE 2.

GSEA of SPTLC123- or DEGS1-silenced cells

Rank	Cluster Key Term	Change	Score	Term
SPTLC123 siRNA GSEA
1	Negative regulation of biosynthetic process	UP	4.02	74
2	Endomembrane system	MIXED	3.28	87
3	Organelle lumen	MIXED	3.10	179
4	Response to extracellular stimulus	MIXED	2.95	35
5	Transcription factor binding	UP	2.91	71
6	Blood vessel development	MIXED	2.51	34
7	Serpin	DOWN	2.34	12
8	Response to organic substance	MIXED	2.29	90
9	Translation regulation	MIXED	2.04	13
DEGS1 siRNA GSEA
1	Endoplasmic reticulum	DOWN	7.33	142
2	Golgi apparatus	MIXED	5.05	122
3	Endomembrane system	DOWN	4.72	114
4	Negative regulation of transcription	UP	3.45	58
5	Organelle lumen	MIXED	3.23	208
6	Positive regulation of cell migration	UP	2.98	20
7	Vesicle	MIXED	2.94	89
8	Transcription repressor activity	UP	2.66	52
9	Cellular response to insulin stimulus	UP	2.62	19
10	Membrane fraction	DOWN	2.55	96
11	Regulation of translation	MIXED	2.50	25
12	Lysosome	DOWN	2.48	26
13	Pleckstrin homology-type	UP	2.30	46
14	Regulation of hydrolase activity	DOWN	2.13	49
15	Establishment of protein localization (transport)	DOWN	2.09	102
16	PDZ (synaptic scaffolding protein)	UP	2.02	24
SPTLC123 and DEGS1 overlap GSEA
1	Negative regulation of cellular biosynthetic process	UP	3.37	37
2	Blood vessel development	MIXED	2.48	18
3	Carboxylic acid / lipid biosynthetic process	DOWN	2.03	14

The clusters based on similarity from DAVID for the gene set enrichment analyses were included in the table if their enrichment score, represented as score in the table, was greater than 2 for each targeted siRNA as well as the overlapping probes that agreed in directionality. The overall change for each process was indicated as UP, DOWN, or MIXED if most (>60%) of the genes represented in the cluster were upregulated, downregulated, or had an undetermined directionality, respectively. The number of terms or genes for each cluster is noted. Gene lists and their directional change for the clusters from the DAVID GSEA can be found in supplementary File IV.