FIG. 2.

Stack plot of in vivo ³¹P NMR spectral time course of encapsulated rat hepatocytes perfused with (A) 20% from 0 to 12 h, (B) 35% from 0 to 27 h (n=2) (C) 50% from 0 to 20 h (n=2), and (D) 95% oxygen from 0 to 27 h (n=2). Each ³¹P NMR spectrum required 68 min to acquire showing every spectrum for the 20% oxygen treatment, and every other spectrum for the other oxygen treatments. NTP, nucleotide triphosphate; DPDE, diphosphodiester; NAD(H), reduced and oxidized nicotinamide adenine dinucleotide; PLI, phospholipid intermediates; Pi_i, intracellular phosphate; Pi_e, extracellular phosphate; SP, sugar phosphates.