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. 2001 Jun 26;98(14):7724–7729. doi: 10.1073/pnas.131059198

Figure 2.

Figure 2

Binding of Pr55Gag and Tsg101 in vitro. (A) Autoradiography to detect immune-captured radiolabeled Tsg101. Lane 1, Tsg101 synthesized in RRL. Lanes 2–9, determination of binding of radioactive Tsg101 to unlabeled Pr55Gag bound to anti-T7 (lane 3), anti-CA (lanes 7 and 8), or anti-p6 (lane 9) IgG immobilized on protein A beads. Antibodies are as defined in Materials and Methods. The amount of RRL used in lanes 2–9 was 5-fold greater than the amount used in lane 1. (B) Confirmation of the presence of Pr55Gag on the beads by Western analysis. A monoclonal antibody against an antigenic site in the CA domain was used to visualize the Gag proteins immunoprecipitated with the antibodies used in A. Molecular mass markers (kDa) are on the left.