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Identification of the region in Tsg101 required for Pr55Gag binding. Truncation (A) and substitution (B and C) mutants of Tsg101 were tested in the two-hybrid assay for interaction with the p1-p6 fusion protein. The figure shows averaged values as a percentage of the wild-type interaction with ±2–6% error. Notations are as in legend to Fig. 1.
