Table 1.
Oligonucleotide Primers for PCR Amplification
| Gene name | Sequence 5′–3′ | Amplified length |
|---|---|---|
| C | JEVC1: 5-ATGACTAAAAAACCAGGAGGG-3 | 381 bp |
| JEVC2: 5-TGGAAATTCGACAACTTCATG-3 | ||
| Prm | JEVP/M1: 5-ATGAAGTTGTCGAATTTCCAG-3 | 501 bp |
| JEVP/M2: 5-CCCATTCCCAGACAATTAAAA-3 | ||
| E | JEVE1: 5-TTTAATTGTCTGGGAATGGGC-3 | 1500 bp |
| JEVE2: 5-TCAATGGCACATCCAGTGTCA | ||
| NS1 | JEVNS12: 5-CTGTTGGCCTCTGCGAAAGCA-3 | 1265 bp |
| JEV NS2A1: 5-GCTTTCGCAGAGGCCAACAGT-3 | ||
| NS2A | JEV NS2A1: 5-GCTTTCGCAGAGGCCAACAGT-3 | 512 bp |
| JEV NS2A2: 5-AACTCAGTAGCTGGCCACCCT-3 | ||
| NS2B | JEV NS2B1: 5-GGGTGGCCAGCTACTGAGTTT-3 | 413 bp |
| JEV NS2B2: 5-GTGTCCCAAAACACGCCCCCT-3 | ||
| NS3 | JEV NS31: 5-GGGGGCGTGTTTTGGGACACG-3 | 1877 bp |
| JEV NS32: 5-TCTATGAAGCTAACGGCTGAT-3 | ||
| NS4A | JEV NS4A1: 5-TCAGCCGTTAGCTTCATAGAG-3 | 821 bp |
| JEV NS4A2: 5-ATGTACCCATAGTGAAGTGTC-3 | ||
| NS4B | JEV NS4B1: 5-ACACTTCACTATGGGTACATG-3 | 431 bp |
| JEV NS4B2: 5-GTCCTGCCCCCAGGCCTTCCC-3 | ||
| NS5 | JEV NS51: 5-GGAAGGCCTGGGGGCAGGACG-3 | 2735 bp |
| JEV NS52: 5-TTCTACCTTAAATCACACTAG-3 |