Figure 1.

Nicotine induces Src phosphorylation in pancreatic adenocarcinoma cells. (A) Expression of α7 subunit of nAChR as seen by reverse transcription-PCR in a variety of pancreatic cancer cell lines. (B) Quiescent L3.6pl metastatic pancreatic cancer cells stimulated with nicotine induce Src phosphorylation within 1 hour. (C and D) Nicotine induces Src phosphorylation in PANC-1 and acquired gemcitabine-resistant L3.6pl^GemRes pancreatic cancer cells with a maximal phosphorylation event at 1 hour with return to baseline within 4 hours.