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. 2012 Dec;14(12):1153–1163. doi: 10.1593/neo.12958

Figure 2.

Figure 2

(A) CITED1 is not detected by immunohistochemistry at e9.5 of murine hepatic development, during the period of hepatic specification as the liver diverticulum (ld) forms (hepatoblasts delaminate) from the ventral foregut endoderm. (B) By e10.5, CITED1 is strongly detected in the proliferating hepatic bud and not detected in the adjacent septum transversum mesenchyme (stm). (C) At e11.5, CITED1 is strongly detected in hepatoblasts, which form hepatic cords (black arrows) following their invasion of the septum transversum mesenchyme. Sinusoids are also seen (white asterisks). (D and E) CITED1 remains strongly detected at e12.5 and e13.5. Hepatoblasts, black arrows; hematopoietic cells, white arrows. (F and G) CITED1 expression begins to taper at e14.5 to e16.5, when CITED1-negative invading hematopoietic cells can be seen (white arrows) and the nuclear-to-cytoplasmic ratio of the hepatoblasts begins to decrease (thin white arrow). (H) CITED1 expression is not detected by e18.5, when hepatic cells have a low nuclear-to-cytoplasmic ratio and have the appearance of fetal hepatocytes [original magnification, x400 (A.H)].