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. 2012 Dec;14(12):1153–1163. doi: 10.1593/neo.12958

Figure 8.

Figure 8

(A) Western blot demonstrates overexpression of CITED1 and detection of epitope-tagged CITED1 (FLAG) in Hep293TT human hepatoblastoma cells transfected with FLAG-tagged wild-type CITED1 (Hep293TT-CITED1) compared to empty vector control plasmid (Hep293TT-pcDNA3). Positive control is MCF7 breast cancer cell line and negative control is COS cell line. (B) qRT-PCR using a Wnt-PCR array revealed a statistically significant increase in fold change of the Wnt inhibitors CXXC4 (log2 fold change = 2.88) and KREMEN1 (log2 fold change = 1.60) and also the Wnt effector DVL2 (log2 fold change = 1.21) after CITED1 overexpression. (C and D) qRT-PCR of human hepatoblastoma specimens reveals a correlation between expression of (C) CITED1 and the Wnt inhibitor CXXC4 (Spearman correlation coefficient = 0.521; P = .024) and (D) CITED1 and the Wnt inhibitor KREMEN1 (Spearman correlation coefficient 0.553; P = .016). (E) No correlation was detected in clinical hepatoblastoma specimens between expression of CITED1 and the KREMEN1 ligand DKK1 (Spearman correlation coefficient = 0.23; P = .342). (F) However, DKK1 was found to be expressed greater in EMEF histology than pure fetal histology hepatoblastoma specimens (P = .003).