Figure 4.

Analysis of cell proliferation within NEB regions under airway repair and steady-state conditions after E-cadherin inactivation. (A) Triple immunofluorescence staining of lung sections for indicated markers. Naphthalene-resistant Clara cells (Clara^V) were identified with their close association with CGRP-expressing neuroendocrine cells in NEBs. Asterisks show proliferating Clara cells that are NEB-independent. Nuclei were counterstained with DAPI (blue). Quantitation of cell proliferation for PNECs (B) and for Clara^V cells (C) for indicated treatment groups. Results are presented as average ± SEM for each group (n = 4). *P < .05.