Figure 6.

Activation of canonical Wnt signaling upon airway injury or as a consequence of dn E-cadherin expression in conducting airway epithelium. (A and B) Immunohistochemistry of lung sections for β-catenin. Duration and type of treatment as indicated. Highmagnification images represent regions shown in insets. Arrows show nuclear staining of β-catenin (brown). Hematoxylin (blue) was used as a counterstain. (C) Semiquantitative RT-PCR analysis of Wnt/β-catenin.regulated genes for control (OFF DOX) and induced (ON DOX) DTR mice. Actin was used as an internal control. (D) Immunoblot analysis of total lung lysates of control and induced DTR mice with the indicated markers.