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. 2012 Dec;14(12):1190–1202. doi: 10.1593/neo.121258

Figure 6.

Figure 6

Depletion of endogenous c-Myc by RNAi enhances Ku DNA binding, DNA-PKcs, and DNA end-joining activities in association with accelerated DSB repair. (A) H460 cells expressing high levels of endogenous c-Myc were transfected with c-Myc siRNA or control siRNA. Expression of c-Myc was analyzed by Western blot. (B–D) Ku DNA binding, DNA-PKcs, and DNA end-joining activities were measured in H460 cells expressing c-Myc siRNA and control siRNA. (E–H) H460 cells expressing c-Myc siRNA or control siRNA were exposed to 5 Gy of IR. After 24 hours, DSBs were measured by PFGE or analysis of γ-H2AX by immunostaining with quantitative evaluation as described in the legend of Figure 2.