Figure 6.

DAB2IP promotes apoptosis in response to IR + NU7441. (A) IR + NU7441-induced apoptosis was determined by DAPI staining. Cells were treated with +/- NU7441 and IR (10 Gy) for 12 hours and the cells were stained with DAPI; the representative fluorescence images are shown. White arrows indicated the mitotic cells and the red arrows represent the apoptotic cells. (B) Quantitative analysis of apoptotic cells. The data are presented as the means ± SD of three independent experiments. (C) Analysis of PARP cleavage. Cells were lysed 12 hours after exposure to IR or IR + NU7441 and subjected to Western blot analysis.